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A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys…

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작성자 관리자 댓글 0건 조회 360회 작성일 23-03-01 00:00

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A Simple and Rapid UPLC-PDA Method for Quality Control of Nardostachys jatamansi


Abstract

Nardostachys jatamansi is a well-documented herbal agent used to treat digestive and neuropsychiatric disoders is oriental medicinal systems. However, few simple, rapid, and comprehensive methods were reported for qiality assess met and control of N.jatamansi. Herein, a UPLC with photodiode array detection method was developed for both fingerprint investigation of N.jatamansi and simultaneous quantitative analysis of six serotonin transporter modulatiry constituents in N.jatamansi. For chromatographic fingerprint, 24common peaks were selected as characteristic peaks to assess the consistnecy of N.jatamansi samples from different retail sources. Six of the common peaks(5, 7, 12, and 16-18) were identified as desoxo-narchinol A, buddleoside, isonardosinone, by pytochemical investigation. Five of the six, compounds significantly either enhanced or inhibited serotonin transporter activity, while(-) aristolone (18) didn't show any compounds showed good linearity (r ＞0.999) within tes ranges. The precision, expressed as relative standard deviation, was in the range of 0.25-2.77%, and the recovery of the method was in range of 92-105%. The UPLC-photodiode array detection-based fingerprint analysis and quantitative methods reported here could be used for routine quality control of N.jatamansi. 

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